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MN #740902.50 NucleoSpin RNA XS, 50 preps

MN #740902.50 NucleoSpin RNA XS, 50 preps
Brand: Macherey-Nagel
Product Code: 740902.50
Availability: In Stock
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NucleoSpin® RNA XS

Total RNA from cells and tissue

5 µL elution volume → highly concentrated RNA
Features 
 
Purification of highly concentrated RNA from smallest samples

• Isolation of RNA from small sample quantities like biopsy material or single cells
• Excellent RNA recovery and integrity
• Concentrated RNA for sensitive downstream applications by elution in as little as 5 μL
• rDNase included for on-column DNA removal
• Efficient homogenization and reduction of viscosity – NucleoSpin® Filters (shredders) included
• High quality RNA, ready to use for RT-PCR and other applications

 

Technology Silica-membrane technology
Format Mini spin column – XS design
Sample material

Small amounts of tissue < 5 mg,
1-100000 cultured cells
Fragment size > 200 b
Typical yield

102 HeLa cells: 0.1–1.5 ng
103 HeLa cells: 10–15 ng
104 HeLa cells: 100–150 ng
105 HeLa cells: 1000–1500 ng
A260/A280 1.9–2.1
Typical RIN (RNA integrity number) > 9 (depending on sample quality)
Elution volume 5–30 µL
Preparation time 40 min/12 preps
Binding capacity 110 µg
 

Applications*
 

• Total RNA isolation from cultured cells
• Total RNA isolation from tissue
• Total RNA isolation from cryosections
• Total RNA isolation from laser captured cells
• Total RNA isolation from small amounts of plant material
• Total RNA isolation from samples stored in RNAlater®
• Typical downstream applications: real-time RT-PCR, Northern blotting, primer extension, array technology,
   RNase protection assays

 

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