MN #740955.50 NucleoSpin RNA II, 50 preps

• Efficient removal of contaminating DNA – rDNase included for on-column DNA digestion*
• Efficient sample homogenization and reduction of viscosity – NucleoSpin® Filters (shredders)
   included
• Up to 70 μg ready-to-use RNA
• Parallel purification of genomic DNA possible by using the NucleoSpin® RNA/DNA Buffer Set

< 5 x 106 cultured cells, 
< 109 bacterial cells, 
< 108 yeast cells, 
< 30 mg tissue

14 μg from 106 HeLa cells, 
70 μg from 109 bacterial cells

Applications**

• Support protocol for total RNA from < 109 bacterial cells (Gram-negative, Gram-positive) or < 108 yeast cells
• Support protocol for total RNA from ≤ 100 μL biological fluids
• Support protocol for RNA clean-up from reaction mixtures
• Support protocol for total RNA from samples stored in RNAlater®

* The amount of DNA contamination is significantly reduced during on-column rDNase digestion. Anyhow, in very sensitive applications it might be possible to detect traces of DNA. The NucleoSpin RNA II system is checked by the following procedure: One million HeLa cells are subjected to RNA isolation according to the protocol. RNA eluate is used as template for PCR detection of a 1 kb fragment in a 30 cycle reaction. Generally, no PCR fragment is obtained if the rDNase is applied. However, a strong PCR fragment is obtained if rDNase is omitted. The eventuality of DNA detection with PCR increases with:
– the number of DNA copies per preparation: single copy target < plastidial / mitochondrial target < cells transfected with plasmid
– decreasing PCR amplicon size

** Kits to be used for research purposes only