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MN #740609.250 NucleoSpin Gel and PCR Clean-up, 250 preps

MN #740609.250 NucleoSpin Gel and PCR Clean-up, 250 preps
Brand: Macherey-Nagel
Product Code: 740609.250
Availability: In Stock
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NucleoSpin® Gel and PCR Clean-up

PCR clean-up / Gel extraction

formerly known as "NucleoSpin® Extract II"
Features 
 
PCR clean-up and gel extraction - the two-in-one kit with optimized recovery and elution volume

• Two applications one kit, one buffer with optimal performance for both applications
• High recoveries for fragments down to 50 bp
• Minimized elution volume: 15 μL → highly concentrated DNA
• Binding buffer with pH indicator
• Separate buffers for single stranded DNA or SDS containing samples available
• Suitable for all gel buffer systems (e.g., TAE, TBE)

  

Technology Silica-membrane technology
Format Mini spin columns
Sample material

< 400 µL PCR reaction mixture 
< 400 mg TAE / TBE agarose gel
Fragment size 50 bp–approx. 20 kbp
Typical recovery 70–95%
A260/A280 1.8–1.9
Elution volume 15–30 µL
Preparation time 10 min/6 preps
Binding capacity 25 µg
 
Applications
 

• Purification of PCR products
• Extraction of DNA from agarose gels
• Support protocol for concentration and desalination of reaction mixtures
• Support protocol for clean-up of single stranded DNA (Binding Buffer NTC required, not included in the kit,
   see “Ordering information”)
• Support protocol for clean-up of SDS-containing samples (Binding Buffer NTB required, not included in the
   kit, see “Ordering information”)
• Typical downstream applications: cloning, sequencing, PCR, restriction analysis

 

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