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MN #740579 NucleoBond BAC 100, 10 preps

Brand: Macherey-Nagel
Product Code: 740579
Availability: In Stock

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NucleoBond® BAC 100	Transfection-grade plasmid DNA
Features
The anion exchanger for purification of P1, BACs, PACs, and other large constructs
• Suitable for constructs up to 300 kbp • Optimized lysis buffer volumes for large bacterial cultures • NucleoBond® Folded Filters included for gentle lysate clarification without shearing of large   constructs

 

 

Technology	Anion-exchange chromatography
Format	Gravity-flow columns
Lysate clarification	Folded filters
Sample material	100–500 mL E.coli culture
Vector size	< 300 kbp
Typical yield	10–100 µg
A260/A280	1.80–1.95
Preparation time	120 min/2–4 preps
Binding capacity	100 µg
Applications
• Purification of P1, BACs, PACs, and other large constructs from E. coli • Typical downstream applications: cloning, PCR, restriction analysis, sequencing, and transfection

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