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MN #740962.20 NucleoSpin RNA L, 20 preps

MN #740962.20 NucleoSpin RNA L, 20 preps
Brand: Macherey-Nagel
Product Code: 740962.20
Availability: In Stock
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NucleoSpin® RNA L

Total RNA from cells and tissue

rDNase and NucleoSpin® Filters included
Features 
 
Midi spin kit for the isolation of RNA of highest integrity

 

• Efficient removal of genomic DNA – rDNase included for on-column digestion*
• Efficient sample homogenization and reduction of viscosity – NucleoSpin® Filters L (shredders)
   included
• Up to 600 μg ready-to-use RNA

  

Technology Silica-membrane technology
Format Midi spin columns
Sample material

< 5 x 107 cultured cells, 
< 1010 bacterial cells, 
< 3 x 108 yeast cells,
< 200 mg tissue
Fragment size 200 b
Typical yield

180 µg from 107 HeLa cells,
620 µg from 4 x 107 HeLa cells
A260/A280 1.9–2.1
Typical RIN (RNA integrity number) > 9
Elution volume 500 µL
Preparation time 80 min/4 preps
Binding capacity 700 µg
 

Applications**
 
• Total RNA isolation from cultured cells, tissue (standard protocol)
• Support protocol for total RNA from < 1010 bacterial cells (Gram-negative, Gram-positive) or 
   < 3 x 108 yeast cells
• Support protocol for RNA clean-up from reaction mixtures
• Support protocol for total RNA from samples stored in RNAlater®
• Typical downstream applications: real-time RT-PCR, Northern blotting, primer extension, 
   array technology, RNase protection assays
 

* The amount of DNA contamination is significantly reduced during on-column rDNase digestion. Anyhow, in very sensitive applications it might be possible to detect traces of DNA. The NucleoSpin RNA L system is checked by the following procedure: One million HeLa cells are subjected to RNA isolation according to the protocol. RNA eluate is used as template for PCR detection of a 1 kb fragment in a 30 cycle reaction. Generally, no PCR fragment is obtained if the rDNase is applied. However, a strong PCR fragment is obtained if rDNase is omitted. The eventuality of DNA detection with PCR increases with:
– the number of DNA copies per preparation: single copy target < plastidial/ mitochondrial target < cells transfected with plasmid
– decreasing PCR amplicon size

** Kits to be used for research purposes only

 

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