MN #740588.250 NucleoSpin Plasmid, 250 preps

MN #740588.250 NucleoSpin Plasmid, 250 preps
Brand: Macherey-Nagel
Product Code: 740588.250
Availability: In Stock
   - OR -   


NucleoSpin® Plasmid

Sequencing-grade plasmid DNA

NucleoSpin® Plasmid (NoLid)  
For rapid small scale preparation of up to 40 μg of high-purity plasmid DNA

• Fast procedure: 18 Mini preps in 25 min
• High capacity: up to 40 μg of plasmid DNA


Technology Silica-membrane technology
Format Mini spin columns
Sample material 1–5 mL E. coli culture (standard protocol)
5–10 mL E. coli culture*
Vector size < 15 kbp
Typical yield < 25 µg (from 1–5 mL E. coli culture)
< 40 µg (from 5–10 mL E. coli culture)
A260/A280 1.80–1.85
Elution volume 50 µL
Preparation time 25 min/18 preps
Binding capacity 60 µg

• High-copy plasmids from E. coli 
• Support protocol for low-copy plasmids, P1 constructs, or cosmids*
• Support protocols for plasmid DNA from Gram-positive bacteria and plasmid DNA clean-up from reaction
• Support protocol for M13 DNA available on request, please contact Technical Service
• Typical downstream applications: cloning, sequencing, PCR, transformation, restriction analysis

* Increased buffer volumes required, see "Ordering information"


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